空氣污染與動物水平研究有關科研的主要方法
- 發(fā)布人：
- 發(fā)布日期：2017-04-21 11:34:18
（一）在體研究：
整體（全身）暴露：被普遍認為是最接近模擬人的現(xiàn)實暴露的方式。對PM2.5暴露來說，目前主要有濃縮暴露系統(tǒng)和非濃縮（現(xiàn)實濃度）暴露系統(tǒng)。代表性參考文獻如下：
濃縮系統(tǒng)：Xu X, et al. Long-term exposure to ambient fine particulate pollution induces insulin resistance and mitochondrial alteration in adipose tissue. Toxicol Sci. 2011;124(1):88-98
非濃縮系統(tǒng)：Xu X, et al. Association of systemic inflammation with marked changes in particulate air pollution in Beijing in 2008. Toxicol Lett. 2012;212(2):147-56
“開放”(Open top)系統(tǒng)：Mauad T, et al. Chronic exposure to ambient levels of urban particles affects mouse lung development. Am J Respir Crit Care Med. 2008;178(7):721-8.
罩棚(shed)系統(tǒng)：Somers CM, et al. Reduction of particulate air pollution lowers the risk of heritable mutations in mice. Science. 2004;304(5673):1008-10
經(jīng)鼻暴露：多為將顆粒物溶入細胞培養(yǎng)液中經(jīng)鼻腔暴露，比較“機械”但仍屬于“經(jīng)氣管”的渠道。代表性參考文獻如下：
Arantes-Costa FM, et al. Effects of residual oil fly ash (ROFA) in mice with chronic allergic pulmonary inflammation. Toxicol Pathol. 2008;36(5):680-6
